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Protein Biochemistry
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Rhodamine Phalloidin (14uM in methanol)
Rhodamine Phalloidin (14uM in methanol) from Cytoskeleton, Inc.
(1)
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Citations:
(127)
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Cytoskeleton, Inc. for
Rhodamine Phalloidin (14uM in methanol)
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Product Specs
Item
Rhodamine Phalloidin (14uM in methanol)
Company
Cytoskeleton, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
PHDR1
Quantity
1 x 500 µl
Brand/Trademark
Cytoskeleton, Inc
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Cytoskeleton, Inc.
1830 S. Acoma St.
Denver
,
Colorado
80223
United States
Phone
: 303 322-2254
303 322-2257
Company Profile
Website:
http://cytoskeleton.com/
Citations (127)
(101)
Antioxidants and NOX1/NOX4 inhibition blocks TGFβ1-induced CCN2 and α-SMA expression in dermal and gingival fibroblasts
PLOS ONE
October 19, 2017
Hannah Murphy-Marshman, Katherine Quensel, Xu Shi-wen, Rebecca Barnfield, Jacalyn Kelly, Alex Peidl, Richard J. Stratton, Andrew Leask
(
715-035-150
) were from
Jackson Immunoresearch Laboratories
. AlexaFluor™-conjugated phalloidin (
1:1000
;
PHDR1
) was from
Cytoskeleton
.
Cell culture
Human
dermal (ATCC) and gingival (a generous gift from Dr. Douglas
(102)
Effect of Fibronectin-Coated Micro-Grooved Titanium Surface on Alignment, Adhesion, and Proliferation of Human Gingival Fibroblasts
Medical Science Monitor
October 4, 2017
Xiaochong Jian, Wenxiu Huang, Dong Wu, Dongdong You, Zhaonan Lin, Jiang Chen
in the dark. Subsequently, the cell samples were stained with rhodanine phalloidin (
1: 400
, Cat. #
PHDR1
,
Cytoskeleton
, Denver, CO, USA) for 30 min in the dark, and then stained with DAPI (
1: 1000
, D9542,
(103)
Non-muscle myosin IIB (Myh10) is required for epicardial function and coronary vessel formation during mammalian development
PLoS genetics
October 1, 2017
Ridge LA, Mitchell K, Al-Anbaki A, Shaikh Qureshi WM, Stephen LA, Tenin G, Lu Y, Lupu IE, Clowes C, Robertson A,
et al
.
30 minutes. Coverslips were washed and then treated with 100nM rhodamine-phalloidin (
Cytoskeleton
,
PHDR1
) for 30 minutes to allow visualisation of the
actin cytoskeleton
. Following a final wash, coverslips
(104)
Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Molecular biology of the cell
September 15, 2017
Long KR, Shipman KE, Rbaibi Y, Menshikova EV, Ritov VB, Eshbach ML, Jiang Y, Jackson EK, Baty CJ, Weisz OA.
mouse
α-acetylated tubulin
1:500
(
Sigma-Aldrich
;
T7451
); rhodamine phalloidin
1:140
(
Cytoskeleton
;
PHDR1
); rabbit anti–megalin
1:1000
(MC-220, gift from Dan Biemesderfer, Yale University [ Zou et al., 2004]);
(105)
Multicolor Stimulated Emission Depletion (STED) Microscopy to Generate High-resolution Images of Respiratory Syncytial Virus Particles and Infected Cells
BIO-PROTOCOL
September 5, 2017
Masfique Mehedi, Margery Smelkinson, Juraj Kabat, Sundar Ganesan, Peter L. Collins, Ursula J. Buchholz
IgG-Atto 647N (
Sigma-Aldrich
, catalog number:
40839
) Rhodamine phalloidin (
CYTOSKELETON
, catalog number:
PHDR1
)
ProLong Gold Antifade Mountant
(
Thermo Fisher Scientific
,
Invitrogen
™, catalog number:
P36930
)
(106)
Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells
Scientific reports
July 18, 2017
Chen YD, Fang YT, Cheng YL, Lin CF, Hsu LJ, Wang SY, Anderson R, Chang CP, Lin YS.
After permeabilization, cells were stained with DAPI and rhodamine-phalloidin (
Cytoskeleton, Inc
.,
PHDR1
) for nucleus and F-actin. Cells were mounted and analyzed by
confocal microscopy
. Statistical analysis Pearson’s
(107)
Biofunctionalized Plants as Diverse Biomaterials for Human Cell Culture
Advanced Healthcare Materials
April 1, 2017
Gianluca Fontana, Joshua Gershlak, Michal Adamski, Jae‐Sung Lee, Shion Matsumoto, Hau D. Le, Bernard Binder, John Wirth, Glenn Gaudette, William L. Murphy
stained using phalloidin. Samples were incubated for 2 hours in rhodamine–phalloidin (
Cytoskeleton Inc
,
PHDR1
) following directions from the supplier’s protocol and 10 min in 4′,6-diamidino-2-phenylindole (DAPI) to
(108)
Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells
Molecular vision
January 1, 2017
Samuel W, Jaworski C, Postnikova OA, Kutty RK, Duncan T, Tan LX, Poliakov E, Lakkaraju A, Redmond TM.
cells were stained with the actin stain rhodamine-phalloidin (
1:200
;
Cytoskeleton
, Denver, CO, Cat #
PHDR1
) and Alexa Fluor secondary antibodies (Thermo Fisher Scientific) at
1:500
in 1% BSA for 30 min. The
(109)
NRF2 promotes breast cancer cell proliferation and metastasis by increasing RhoA/ROCK pathway signal transduction
Oncotarget
November 8, 2016
Zhang C, Wang HJ, Bao QC, Wang L, Guo TK, Chen WL, Xu LL, Zhou HS, Bian JL, Yang YR,
et al
.
formation
assays
, the cells were stained with
Rhodamine Phalloidin
for 30min (
Cytoskeleton
, Cat No.
PHDR1
). The coverslips were mounted in Vectashield mounting medium with DAPI. The
fluorescence
images were
(110)
Berberine induces autophagy in glioblastoma by targeting the AMPK/mTOR/ULK1-pathway
Oncotarget
October 11, 2016
Jiwei Wang, Qichao Qi, Zichao Feng, Xin Zhang, Bin Huang, Anjing Chen, Lars Prestegarden, Xingang Li, Jian Wang
light microscope. The
cytoskeleton
changes in U251 and U87 were visualized using rhodamine phalloidin (
PHDR1
,
Cytoskeleton, Inc
.) which was performed according to the manufacturer's protocol, and the cells were
(111)
Nitidine chloride inhibits the malignant behavior of human glioblastoma cells by targeting the PI3K/AKT/mTOR signaling pathway
Oncology Reports
October 1, 2016
Ming Liu, Jiwei Wang, Qichao Qi, Bin Huang, Anjing Chen, Xingang Li, Jian Wang
anti-rabbit IgG (
Santa Cruz Biotechnology
). Cells were incubated in the dark with Rhodamine phalloidin (
PHDR1
;
Cytoskeleton, Inc
., Denver, CO, USA) and DAPI to stain actin and nuclei, respectively. Slides were
(112)
Protective responses to sublytic complement in the retinal pigment epithelium
Proceedings of the National Academy of Sciences of the United States of America
August 2, 2016
Tan LX, Toops KA, Lakkaraju A.
(
1:100
; Novus), and ZO-1 (
1:3000
) (25). Other reagents were as follows: rhodaminephalloidin (
1:200
,
PHDR1
;
Cytoskeleton
), Alexa-conjugated secondary antibodies (
1:500
), and DAPI (
1:200
), both from ThermoFisher. PNAS
(113)
Effects of testosterone on the expression levels of AMH, VEGF and HIF-1α in mouse granulosa cells
Experimental and Therapeutic Medicine
August 1, 2016
Ying Zhang, Shan-Feng Wang, Jin-Dan Zheng, Chun-Bo Zhao, Yi-Nan Zhang, Li-Li Liu, Jian-Hua Huang
for 5 min. For F-actin
staining
, the cells were incubated with 2 U/ml rhodamine-phalloidin (cat. no.
PHDR1
;
Cytoskeleton, Inc
., Denver, CO, USA) for 1 h at room temperature in the dark, and were mounted using
(114)
Reduced Expression of Galectin-9 Contributes to a Poor Outcome in Colon Cancer by Inhibiting NK Cell Chemotaxis Partially through the Rho/ROCK1 Signaling Pathway
PLOS ONE
March 30, 2016
Yang Wang et al.
Triton X-100
(X100,
Sigma-Aldrich
, USA). The cells were then incubated with rhodamine-labeled phalloidin (
PHDR1
,
Cytoskeleton
, USA) and DAPI (C1002, Beyotime Biotechnology, Shanghai, China) according to the manufacturer’s
(115)
SALL4 is a marker of poor prognosis in serous ovarian carcinoma promoting invasion and metastasis
Oncology Reports
March 1, 2016
MENGYUAN YANG, XIAOHUI XIE, YILING DING
to block unspecific binding of the antibodies, and then incubated with 100 nM rhodamine phalloidin (#
PHDR1
;
Cytoskeleton, Inc
., Denver, CO, USA) at room temperature in the dark for 30 min. After three washes,
(116)
Scutellarin regulates the Notch pathway and affects the migration and morphological transformation of activated microglia in experimentally induced cerebral ischemia in rats and in activated BV-2 microglia
Journal of Neuroinflammation
December 1, 2015
Yun Yuan, Parakalan Rangarajan, Enci Mary Kan, Yajun Wu, Chunyun Wu, Eng-Ang Ling
assay
F-actin
labeling
To detect the
actin cytoskeleton
in
microglia
, Rhodamine Phalloidin (3.5:500, Cat.
PHDR1
,
Cytoskeleton Inc
., USA) was used. BV-2
microglial cells
grown on poly-L-lysine coated cover slips were
(117)
Inhibition of microtubule-associated protein 1 light chain 3B via small-interfering RNA or 3-methyladenine impairs hypoxia-induced HO8910PM and HO8910 epithelial ovarian cancer cell migration and invasion and is associated with RhoA and alterations of the actin cytoskeleton
Oncology Reports
March 1, 2015
ZHONGYUAN TANG, NING ZHANG, WEN DI, WEIPING LI
monoclonal anti‑ras homolog gene family, member A (RhoA) antibody (no.
ARH03
) and rhodamine phalloidin (no.
PHDR1
) were purchased from
Cytoskeleton Inc
. (Denver, CO, USA). The
Rho Assay Reagent (Rhotekin RBD, agarose
;
(118)
Cholesterol-mediated activation of acid sphingomyelinase disrupts autophagy in the retinal pigment epithelium
Molecular biology of the cell
January 1, 2015
Toops KA, Tan LX, Jiang Z, Radu RA, Lakkaraju A.
Alexa Fluor secondary antibodies were used at
1:500
and rhodamine–phalloidin (
Cytoskeleton
, Denver, CO;
PHDR1
) at
1:200
. Filters were mounted under coverslips on glass slides under Vectashield (Vector Labs, Peterborough,
(119)
Sox4-mediated caldesmon expression facilitates differentiation of skeletal myoblasts
Journal of Cell Science
November 15, 2013
Sang-Min Jang, Jung-Woong Kim, Daehwan Kim, Chul-Hong Kim, Joo-Hee An, Kyung-Hee Choi, Sangmyung Rhee
(Sigma) was used for nuclear
staining
. Rhodamine-Phalloidin was used for polymerized actin
staining
(
PHDR1
;
Cytoskeleton
). Samples were incubated with 300 nM DAPI in PBS for 2 minutes at room temperature and visualized
(120)
LMX1B is Essential for the Maintenance of Differentiated Podocytes in Adult Kidneys
Journal of the American Society of Nephrology
November 1, 2013
Tillmann Burghardt et al.
Total cell area was determined by
staining
the
actin cytoskeleton
with rhodamine phalloidin (diluted
1:300
;
PHDR1
;
Cytoskeleton
). Digital pictures of the green and red channels were quantitated using the ImageJ software
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Reviews
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(1)
2/11/2019
Rhodamine Phalloidin for Actin Staining
Dr. Mohammad Alam
Our aim was to examine F-actin staining in cardiomyocytes. We transfected adult cardiomyocytes with the scramble and target specific siRNA. It was analyzed with F-actin specific antibody. Antibody is...
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Rhodamine Phalloidin (14uM in methanol) from Cytoskeleton, Inc.
Supplier Page
View Company Product Page
Pricing Info
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